Recombinant Human FGF-4功能與作用機(jī)制

種屬：Human

規(guī)格：10μg/50μg/1mg

純度：≥95%

內(nèi)毒素： ≤10 EU/mg

表達(dá)系統(tǒng)：E. coli

功能與作用機(jī)制

FGF-4是一種有效的促有絲分裂原和形態(tài)發(fā)生素。其作用具有情境依賴性，因細(xì)胞類型、濃度以及其他因子的存在而異。

1、維持多能性與啟動(dòng)分化： 在胚胎干細(xì)胞和誘導(dǎo)多能干細(xì)胞中，FGF-4有助于維持多能性，并啟動(dòng)細(xì)胞向特定譜系（特別是中胚層譜系）分化。

2、肢芽發(fā)育： 這是其在胚胎發(fā)育中的經(jīng)典作用。FGF-4在肢芽的頂端外胚層嵴中表達(dá)，通過刺激下方間充質(zhì)細(xì)胞的增殖，對肢體的生長和模式形成至關(guān)重要。

3、中胚層分化： 驅(qū)動(dòng)多能細(xì)胞向中胚層祖細(xì)胞分化，這些祖細(xì)胞可進(jìn)一步分化為心肌細(xì)胞、肌肉、骨骼和結(jié)締組織。

4、細(xì)胞存活與增殖： 促進(jìn)多種細(xì)胞類型（包括成纖維細(xì)胞、內(nèi)皮細(xì)胞和成肌細(xì)胞）的生長和存活。

(純度) Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).

(內(nèi)毒素) Endotoxin

≤10 EU/mg by the LAL method.

(生物活性) Bioactivity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1 ng/mL.

(檢索號) Accession

P08620

(種屬) Species

Human

(表達(dá)系統(tǒng)) Expression System

E.Coli

(預(yù)測分子量大小) Mol. Weight

19.3 kDa

(表達(dá)區(qū)間) Expression Region

Ala31-Leu206

(標(biāo)簽) Tag Info

Tag free

(劑型) Form

Lyophilized

(溶液) Buffer

Lyophilized from a 0.22 μm filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.

(復(fù)溶) Reconstitution

It is recommended to redissolve in sterile deionized water.

(運(yùn)輸) Shipping

The product is shipped with blue ice.

(存儲(chǔ)) Storage & Stability

12 months at -20℃ to -80℃in lyophilized state;

6 months at -20℃ to -80℃ under sterile conditions after reconstitution;

7-10 days at 2℃ to 8℃under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.